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KMID : 1140120100150040268
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Cancer Prevention Research
2010 Volume.15 No. 4 p.268 ~ p.276
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Generation of Tumor Specific Human Antibody Fragment, LA-calu-1, Selected with Lung Adenocarcinoma Cell Line, Calu3
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Han Il-Yong
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Abstract
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For developing the new methods of treatment and diagnosis, lung adenocarcinoma specific human monoclonal antibody was generated using the human naive antibody phage display library. By the subtractive cell panning, phage antibodies that react to lung adenocarcinoma cell line, Calu3, but not react to normal lung epithelial cell, Wi-26, were selected. Reactivity to Calu3 of selected clones was tested with flow cytometry. Selected clones were analyzed with restriction enzyme and nucleotide sequencing. Tissue specificity and tumor specificity was tested with flow cytometry. Four clones were selected by flow cytometry analysis of 60 clones rescued after 4th panning. By the BstN1 fingerprinting and nucleotide sequencing, 4 clones were same clone, so it named to LA-calu-1. LA-calu-1 react to liver and lung origin cells, but not react to normal cell lines, LS513 and Wi-26. In this study, I generated LA-calu-1 that reacted to hepatocarcinoma and lung adenocarcinoma cell lines. In further study, I will confirm the probability of application in cancer treatment and diagnosis.
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KEYWORD
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Carcinoma, Non-small cell, Lung, Antibody formation, Tumor specific human monoclonal antibody
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